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KMID : 1134820220510070640
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Journal of the Korean Society of Food Science and Nutrition
2022 Volume.51 No. 7 p.640 ~ p.650
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Effect of Eurycoma longifolia Extract on Testosterone Synthesis in TM3 Leydig Cells under Oxidative Stress
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Jung Jae-In
Eun Sang-Won
Lee Jae-Kyoung
Seo Young-Ha
Bae Mun-Hyoung
Kim Ryong
Kim Eun-Ji
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Abstract
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Animal and human studies have suggested that Eurycoma longifolia extract (ELE) ameliorates andropause symptoms by increasing testosterone levels. However, the mechanisms responsible for this effect have yet to be elucidated. We examined the effects of ELE on testosterone levels and the expressions of steroidogenic enzymes in TM3 Leydig cells under H2O2-induced oxidative stress. When cells were treated with 50 μM H2O2, testosterone levels and the expressions of steroidogenic acute regulatory protein (StAR) (involved in cholesterol transport) and 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17-α-hydroxylase (CYP17A1) (involved in testosterone synthesis) were significantly decreased. On the other hand, the expressions of 5α-reductase and aromatase, which convert testosterone to dihydrotestosterone and estradiol, respectively, were increased. In addition, ELE significantly increased testosterone levels and the expressions of StAR, 3β-HSD, and CYP17A1, but decreased 5α-reductase and aromatase expressions in TM3 cells exposed to H2O2-induced oxidative stress. These results indicate that ELE protects against oxidative stress-induced testosterone reduction by increasing cholesterol transport and testosterone synthesis and decreasing testosterone conversion. These observations suggest ELE might be used to alleviate the symptoms of andropause caused by low testosterone levels.
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KEYWORD
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Eurycoma longifolia, Leydig cells, testosterone, oxidative stress, steroidogenic enzyme
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